FAN Yin-qiu¹，CUI Jing-cheng¹，YANG Jun²，WANG Pin³

（1 The Department of Postgraduate Education，Anhui University of Traditional Chinese Medicine，Hefei 230038；2 1st Affiliated Hospital，Anhui University of Traditional Chinese Medicine，Hefei 230038；3  Anhui University of Traditional Chinese Medicine，Hefei230038）

Abstract: Objective:  To discuss the effect of Huayutongluo Moxibustion on the delayed memory of  vascular  dementia  rats  and  the  expression  of  Nestin 、DCX  in  the  hippocampus  after  the transplantation of NSCs+EPCs  and then illustrate the relationship with neurogenesis.  Methods:Male  Wistar  rats  of  SPF  level  were  randomly  separated  into  control  group ，model  group，NSCs+EPCs  moxibustion  group  and  NSCs+EPCs  control  group.  Improved  2-VO  method  was used  to  make  the  vascular  dementia  models.  The  lateral  ventrcle  of  rats  in  NSCs+EPCs moxibustion  group  were  transplanted  into  NSCs+EPCs  after  3  days  of  models’   identification. Moxibustion was administered once daily for 7 consecutive days and had a rest for one day on  rats in NSCs+EPCs moxibustion group.  7 days comprised a course of treatment and 3 courses  were needed for treating. The Morris Water Maze was used to test the  delayed memory of  rats before and after 24 hours of the third course of treatment. The expression of Nestin and DCX was tested with double-labeled immunofluorescence technology.  Results:  The Morris Water Maze shows that the latency  of all groups’   rats  was definitely shorter than period before treatment （P<0.05） besides the VD model group （P＞0.05）， times of crossing the platform of NSCs+EPCs moxibustion group were  definitely  increased  than  period  before  treatmentin  120s （ P<0.05 ） .  The  latency  of NSCs+EPCs moxibustion group was definitely shorter than model group but the times of crossing the platform  were definitely increased than model group（P＜0.008）.The latency of NSCs+EPCs moxibustion group was definitely shorter than model group and NSCs+EPCs control group（P＜0.008）.The immunofluorescence shows that the positive expression in NSCs+EPCs moxibustion group′s rats was more than that in NSCs+EPCs control group （P＜0.05） .The expression of Nestin and  DCX was respectively  highest after  the  1st and 2nd  course of treatment （P＜0.05， P＜0.05） . 

Conclusion:The delayed memory  and the  expression of Nestin  and  DCX of vascular dementia rats after transplanting NSCs+EPCs  can be increased by  Huayutongluo Moxibustion  so that can promote neurogenesis.

Key  words:  Huayutongluo  Moxibustion,  V ascular  dementia,  Hippocampus,  Delayed  memory, Nestin, DCX