ZHANG Tingzhuo, DU Xiaozheng, WANG Jinhai, TIAN Liang ,YUAN Bo, LI Xinglan, ZHANG Fenfan, QIAO Xiang, WANG Yixin

(1 School of Acu-moxibustion and Massage, Gansu University of Traditional Chinese Medicine, Lanzhou

730000; 2 Department of Traditional Chinese Medicine, the Second Hospital of Lanzhou University,

Lanzhou 730030)

Abstract: Objective: To detect the expression of scalp-acupuncture on the expression of IL-6 and IL-10 of hippocampal  CA1  region  in  cerebral  ischemia  (CI)  rats,  so  as  to  investigate  its  mechanism  underlying regulation  of Inflammation  of ischemia stroke.  Methods: 40  SD rats were randomly allocated to normal group, model group,  scalp-acupuncture group  and  drug  group(n=10  in  each group).  The model of  focal  CI  was established  by  middle  cerebral  artery  occlusion(MCAO).  Scalp-acupuncture  stimulation  was  applied  to bilateral  Dingnieqianxiexian  (MS6) and Dingniehouxiexian  (MS7)  once daily for 7 days. Drug group  were injection of pyrrolidine dithiocarbamate 100mg.kg-1/d, once daily for 7 days. The expression of IL-6 and IL-10 was detected by immunohistochemistry. Results: Compared with the normal group the expression of IL-6 and  IL-10  in the hippocampal CA1 region significantly  increased  in the model group (P<0.05),  while the expression of IL-6 in the hippocampal CA1 region obviousiy decreased in both scalp-acupuncture and drug group compared with the model group but the expression of IL-10 increased(P<0.05); Compared with drug group,  the  expression  of  IL-6  and  IL-10  in  hippocampal  CA1  neurons  of  rats  was  not  statistically significant(P>0.05).  Conclusion:  Scalp-acupuncture  stimulation  can  increase  the  expression  ofantiinflammatory factor IL-10,  while  decrease  the expression of  inflammatory factor IL-6  of ischemia stroke,which may be one of the molecular mechanisms of acupoint stimulation of acute ischemic cerebrovascular disease.

Key Words: Scalp-acupuncture, Ischemic stroke, IL-6; IL-10, Inflammatory response