SONG Chunhua, DING Xu, WANG Baoye, FU Jiaming, XU Hongfei, GONG Weizhi, DONG Qinjian,  LI Xiaoyan, GUO Dan, JIN  Yan-Hong,  YAN Jun,KANG Lianru

（1 Heilongjiang University Of Chinese Medicine, Heilongjiang Harbin 150040；2 Yilongxian people's hospital,   Yilong 637600）

Abstract:  Objective:  To  investigate  the  molecular  mechanism  by  study  of  Xinshu  electrical acupuncture preconditioning  on  the  open  regulation  mechanism  of  mitochondrial  permeability transition pore in rat ischemic myocardium  .  Methods:  Rats divided into blank group, model group, xinshu acupuncture preconditioning group , ischemic preconditioning group by random number table  corresponding.  Duplicate  myocardial  ischemia  and  reperfusion  injury  model  by  alive  rat heart  ligation  of  left  anterior  descending  coronary  artery .  Blank  group:  Rats  are  fixed  with acupuncture preconditioning group for 30 minutes but not acupuncture before molding;once per day,continue 15 days. Model group:  Rats are fixed with blank group before molding; No other factors  were  imposed  on  the  whole  experiment  except  myocardial  ischemia reperfusion.Acupuncture preconditioning group: Acupuncture 15 days until molding; once per day, 30 min every time,then MIRI molding.Ischemic preconditioning group: Dealing with rats the same as  the  blank  group  before  molding; To block  blood  flow 5  min  then  perfusion  5  min   before coronary  artery  ligation  on  molding.  Repeated  3  times  in  a  row,  a  total  of  30  min  ischemic preconditioning.  Detect  myocardial  cell  mitochondrial  membrane  permeability  transition  pore opening degree after myocardial ischemia molding success 0 h, 24 h, 48 h. Results: 1. The model group  dyed  myocardial  mitochondria  green  fluorescent  compared  with  the  blank  group  was reduced obviously by laser confocal microscope observing, the experiment result shows that the mitochondrial membrane permeability transition pore activity of model group was significantly reduced  (P < 0.01). 2. Ischemic  preconditioning  0h  group  and  acupuncture  preconditioning  0h group  mitochondrial  fluorescence  intensity  is  significantly  reduced  compared  with  the  model group by laser confocal microscope observing, but the indicators have no significant difference between  ischemic  preconditioning  0h  group  and  acupuncture  preconditioning  0h  group  (P> 0.05).3. Acupuncture preconditioning 24h, 48h group mitochondria fluorescence intensity have a certain enhancement (P < 0.05 or P<0.01) observed by laser confocal microscope compared with ischemic  preconditioning  group  and  acupuncture  preconditioning  0h  group.4.  Experiment  data shows  that  acupuncture  preconditioning  group  and  ischemic  preconditioning  group  both  can reduce myocardial ischemia reperfusion injury; And perhaps acupuncture preconditioning 24  h group is the best treatment plan.  Conclusion:  1. The protection of electrical acupuncture Xinshu preconditioning for myocardial ischemia after 0h,24h,48h was different, the therapy effect enhance - peak - attenuated trend increased with time,and perhaps acupuncture preconditioning 24 h group is  the  best  treatment  plan.2.Electrical  acupuncture  Xinshu  has  protective  effect  for  MIRI  ,the mechanism may be by inhibiting mPTP opening in myocardial ischemia reperfusion injury and reducing the mitochondrial apoptosis then reduce the myocardial ischemia injury.

Key words: Acupuncture and massage, Acupuncture preconditioning,  Ischemic preconditioning, The laser confocal microscope, Mitochondrial permeability transition pore