世界针灸学会联合会

STAT5 is involved in Remote Ischemia Preconditioning and Electro-acupuncture Pretreatment on Myocardial Ischemia/Reperfusion Injury in Mice

author:孙梦晓 source:本站原创 Click:287 update:2018-04-27
  

CHEN Hui , JIN Xin-Yue , SHEN  Yu-Jun , W ANG Tian-Lin , OU Chen , LUSheng-Feng , CAI  Yun , LI Qian , DING  Ya-Juan   and ZHU Bing-Mei

(1 Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, Nanjing 210023, China, 2 Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China 610041)

Abstract Background: To investigate whether Stat5 is a true player for cardioprotective effect on remote ischemic preconditioning (RIPC), and explored the role of Stat5 in electro-acupuncture (EA) pretreatment against myocardial I/R, and determine  its molecular mechanisms.  Methods: Stat5fl/flmice and Stat5-cKOmice  were  randomly  divided  into  6  groups:  Stat5fl/fl+I/R  and  Stat5-cKO+I/R  groups  (30/180  min  left coronary  artery  occlusion/reperfusion);  Stat5fl/fl+RIPC+I/R  and  Stat5-cKO+RIPC+I/R  groups  (applied RIPC with three 5-min cycles of femoral artery occlusion and reperfusion before I/R); Stat5fl/fl+EA+I/R and Stat5-cKO+EA+I/R groups (subjected to 20 min/day of EA treatment for 1 week before I/R). The heart tissue and blood were harvested at the end point of I/R, and the parameters were measured.  Results: RIPC activated  STAT5  in  the  heart  subjected  to  I/R.  Decreased  expressions  of  Cyt,  cleaved  caspase-3,  and increased Bcl-xL and Bcl-2 were detected only in the presence of STAT5.RIPC increased cardiac HIF-1α, IL10, p-PI3K, and VEGF proteins only in the Stat5fl/flmice with RIPC. EA reduced myocardial infarct size and TUNEL-positive  cells  in  both  presence  and  absence  of  STAT5,  suggesting  its  Stat5-independency. RNA-seq data further provided evidence that both RIPC and EA pretreatment activated multiple pathways related their protective role against myocardial I/R injury.  Conclusion: RIPC-induced protection against myocardial I/R injury was Stat5 dependent, and was through anti-apoptotic signaling and survival signaling. The cardioprotection induced by EA was Stat5-independent, but Stat5 does participate in the event through anti-apoptotic and survival signaling at certain extent.

Key words:  Acupuncture, RIPC, Electro-acupuncture pretreatment, Stat5