世界针灸学会联合会

Effect of Electroacupuncture on Expression of High Mobility Group Protein 1 in the Hippocampus in Rats with Chornic Neuropathic Pain

author:孙梦晓 source:本站原创 Click:279 update:2018-04-28
  

WANG Ke,ZENG Liang,ZHOU Yiren,ZHOU Yinfan,ZHAO Ran,YANG Liping,HOU Wenguang

(Research Institute of Acupuncture Anesthesia,Shu Guang Hospital Affiliated to the Shanghai University of Traditional Chinese Medicine,Shanghai 201203,Department of Acupuncture-Moxibustion,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)

Abstract: Objective:  To observe the effect of electroacupuncture (EA) on expression of High Mobility  Group  Protein  1  (HMGB1)  and  related  downstream  effectors  of  proinflammatory cytokines in the hippocampus in chronic neuropathic pain rats, so as to investigate the mechanism of EA analgesia. Methods: Male Sprague-Dawley rats were randomized into sham group, model group, and EA group, with 12 rats in each group. The chronic constriction injury (CCI) model was established by ligation of the left sciatic nerve. EA was applied to bilateral “Zusanli”(ST 36) and “Yanglingquan”(GB 34) for 30 min, once daily for 7 days. The withdrawal mechanical threshold (WMT) was detected in left hind paw. The expression level of HMGB1 in the hippocampus was determined using quantitative RT -PCR and western blotting, and the content of TNF-α and IL-1β were detected by ELISA. Results:  Compared with the sham group, the MWT values at different time point were decreased markedly after modeling (P < 0.001). After EA intervention for 3 and 7 days, the MWT values were up-regulated in EA group (P < 0.05, P < 0.01).   Compared with the sham group, the mRNA and protein expression levels of HMGB1 were increased markedly (P  < 0.001), while the protein expression levels of TNF-α and IL-1β were also up-regulated (P  < 0.001). After EA intervention, the levels of HMGB1 mRNA and protein expression were notably downregulated in the EA group than in the model group (P < 0.001, P < 0.01), while the levels of TNF-α and IL-1β were also significantly down-regulated (P  < 0.05). Conclusion: EA stimulation can relieve  the  chronic  neuropathic  pain,  which  may  be  related  with  inhibition  of  HMGB1  and 

associated downstream effectors of proinflammatory cytokines in the hippocampus.

Key words:  Acupuncture and moxibustion, Electroacupuncture analgesia, Chronic neuropathic pain, Hippocampus, High Mobility Group Protein 1