XIA Liang-jun, HU Yu-Jiao, Yao Bing, Chen Li , Cheng Qian , Cheng Jie, XIA You-bing

(1.Nanjing Medical University,Nanjing 210029,Jiangsu,China;2.Center for Reproductive Medicine,Nanjing General Hospital of Nanjing Military Region,PLA,Nanjing 210002,Jiangsu,China;3.Nanjing University of CM,Nanjing 210023,Jiangsu , China)

Abstract：Objective：To observe the effect of electroacupuncture and bone marrow mesenchymal stem cells transplantation in the treatment  of thin endometrium and its possible mechanism.   Methods：Fifty clean grade adult SD rats were randomly divided into 5 groups: normal group, model group, bone marrow mesenchymal stem cells group（BMSCs）, electroacupuncture group,combined group.Each group of 10. In addition to the normal group, the remaining four groups of rats were used in the estrus 95% ethanol intrauterine injection method to establish a thin endometrial model. The model group was injected with PBS  solution  after  modeling.  BMSCs  were  injected  into  the  stem  cell  suspension  after modeling.Electroacupuncture  group  in  the  modeling  the  next  day  from  the  needle  "Guan  Yuan", "Sanyinjiao", "uterus".The rats in the combined group in addition to injection of cell suspension and were treated  with  electroacupuncture  at  the  time  of  modeling. At  three  emotional  cycle  after modeling,Hematoxylin-eosin staining was used to observe the changes of endometrial tissue morphology, the  thickness  of  endometrium  and  the  number  of  vascular  glands,The  expression  of  vimentin  and cytokeratin was detected by immunohistochemistry.The expression of LIF and hoxa-10 was determined by western blot assay.  Results：①  Compared with the model group, the endometrium of BMSCs group, electroacupuncture  group  and  combined  group  had  different  degrees  of  thickening,  the  difference  was statistically significant(P <0.05). The intimal thickness of the combined group was the closest to the blank group.②  Compared with the model control group, the number of endometrial blood vessels and glands in each group increased in different degrees (P<0.05), and the intimal tissues were complete and regenerated to a certain extent.  ③  The expression of vimentin and cytokeratin was the strongest in the normal group and the weakest in the model group. Electroacupuncture group, BMSCs group and combined group were sequentially  enhanced(P  <0.05). ④ The  expression  of  LIF  and  hoxa-10  protein  in  each  group  was significantly higher than that in model group (P  <0.01). The change of LIF and hoxa-10 protein was the most  significant  (P  <0.05).    Conclusion：Bone  marrow  mesenchymal  stem  cell  transplantation  and electroacupuncture can promote the regeneration and repair of thin endometrium in rats, And the combined use of both methods to show a better trend.

Key words:  Electroacupuncture intervention,  bone  marrow  mesenchymal stem cells,  Thin endometrium, Endometrial receptivity