世界针灸学会联合会

Electroacupuncturing jiaji points prolongs the lifespan of ALS-SOD1G93A mice

author:孙梦晓 source:本站原创 Click:204 update:2018-05-02
  

(1  2nd Affiliated Hospital of Heilongjiang University of Traditional Chinese  Medicine, Harbin 150001,China;  

2 Heilongjiang University of Traditional Chinese Medicine, Harbin 150040,China )

AbstractObjective:  Electroacupuncturing  jiaji  points(ex-b2)  has  been  used  in  ALS  patients  clinically  in China.  Data  shows  that  the  motor  funtion  of  ALS  patient  improved  significantly  by  this  therapy.  To investigate the treatment effects, include onset, survival and motor function, etal., a control experiment was performanted to test the effects of  electroacupuncture at jiaji points(ex-b2) of ALS SOD1G93A mice. 

Methods: ALS SOD1 G93A transgenic mice were treated by electroacupuncture at jiaji points (ex-b2) with the electrial stimulation  at the frequency of 2HZ from the age of d60, 20min/time, 2 times/week for 4 weeks. The treatment effet was compared with the control group disposed by binding, seizing and nipping the mouse hair at the jiaji points(ex-b2) at the same time. In this study, we proceeded Tail Suspension Test, Turn-club Test to investigated the onset of the disease,survival time of the mice and motor function during the process of the disease, 2 times/week from the age of d60 to the end event occurrence. All the data  obtained  through  aforementioned  procedure  was  analyzed  by  the  using  of  SPSS  17.0,  gaussian distribution was test first for every group.Data which is in accordance with normal distribution proceeded with independent-samples t test subsequently, and data that is not conform to the normal distribution were going on with rank sum test. Results of the analysis presented as mean value±standard deviation;KaplanMeier  Survival  analysis  was  proceeded  to  analyze  the  survival  datas.  We  setted  p<0.05  stands  for  the 

difference has significant meaning. Results: 1. Analysis of onset and survival time: EA group shows the delay of  the  onset  of  the  disease  for  9  days  compared  with  the  control  group,  the  difference  has  significant meaning(p<  0.01), and the EA group shows a prolong of the survival time of ALS mice for 7 days compared with  the  control  group,  the  difference  has  significant  meaning(p<0.01).    2.  Analysis  of  Weight:Data shows that mice weight of EA group was higher than that of the control group from the age of week21, difference has significant meaning(p<0.05).    3. Analysis of Turn-club Test:residence time has declined since the mice age of week16 in control group, and since the mice age of week17 in EA group.residence time of EA group is significantly longer than that of the control group from the age of week16,difference has  significant meaning(p<0.05). Conclusion: 1)Electroacupuncture  at  jiaji  points(ex-b2)delays  the onset  of  the  disease  and  prolong  the  survival  time  of  the  ALS  SOD1G93A transgenic  mice. 2)Electroacupuncture at jiaji points(ex-b2) improve motor function of the ALS SOD1G93A transgenic mice. 

Key words: Electroacupuncture, Jiaji points(ex-b2), Amyotrophic lateral sclerosis, Survival time