Experimental Study on the Effect of Jiaji Electroacupuncture Combined with Methylprednisolone on Neurotoxicity-related Proteins in Rats with Acute Spinal Cord Injury
SUN Zhong-ren, LI Xiao-ning, FU Hao, ZENG Xiang-xin, WU Lei, CHI Lei.
(1 Heilongjiang University of Chinese Medicine, Harbin 150001,China; 2 Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150001）
ABSTRACT: Objective To observe the effect of electric acupuncture combined with methylprednisolone on the expression of NMDA-NR1 and cleaved caspase-3 protein in spinal cord of rats with acute spinal cord injury. Methods: 120 female Wister rats were randomly divided into normal group, Sham operation group,acute spinal cord injury group,EA+MP group and MDL28170 group.Each group was subdivided into 1d, 3d, 7d and 14d subgroups.Rats with BBB score of 1-3 were selected and the rats were treated for 14 days.After treatment,remove the injured spinal cord and use Western-blot to test the expression of NMDANR1 and cleaved caspase-3. Results:The BBB score shows that EA + MP group rats limb movement function score increases, it has significant difference (P < 0.05).Western blot test shows that the expression of NMDANR1 and cleaved caspase-3 has decreased. Conclusion Electrical acupuncture with MP can improve the ASCI rat clip ridge movement function score, at the same time reduce the expression of NMDA-NR1 and cleaved caspase-3.
Keywords: Acu-moxibustion and Tuina, Jiaji electroacupuncture, Acute spinal cord injury, Methylprednisolone, Neurotoxicity-related