HUANG Yan，WANG Yanping，ZHANG Weibo，JIA Shuyong，LI Hongyan

（1.School of Acu-moxibustion and Tuina，Beijing University of Chinese Medicine，Beijing 100029，China；2.Institute of Acu-moxibustion，China Academy of Chinese Medical Sciences，Beijing 100700，China）

Abstract:  Objective: To establish a more stable pathological model of blocked channel by ligation in rats.  Methods:48  male  SD  rats  were  randomly  divided  into  4  groups  (n  =  12).  The  Ren  meridian  (RN)  low resistance line was measured first. Ligation group 1 ligated 1 point on the RN line; ligation group 2 ligated 2 point on the RN line; ligation group 3 ligated 3 point on the RN line; Ligation group 4 ligated 1 point out of the RN line, the ligation width was 1cm, and the ligation interval was 1-2mm. The interstitial fluid pressure wave  propagation  method  was  used  to  measure  the  amplitude  of  the  interstitial  fluid  pressure  wave propagation  of  the  groups  before  and  after  the  ligation.   Results:The  change  rate  of  interstitial  fluid pressure  wave  before  and  after  ligation  was  8.17%,  -7.83%,  -13.27%  and  -52.80%  respectively  in  four groups.There  was  no  significant  difference  between  the  ligation  group  4,  the  ligation  group  1  and  the ligation group 2 after ligation, but the change rate of the three groups increased from positive to negative. The amplitude of the ligation group 3 was significantly reduced (P<0.01) after ligation compared to before ligation. Conclusion:The ligation method can establish a stable pathological model of channel blockage.

Key words: Meridian;Ligation; Channel Blockage; Rats; Interstitial Fluid Pressure Wave