Preliminary Study on Establishment of Pathological Channel Blockage Model in Rats by Ligation
HUANG Yan，WANG Yanping，ZHANG Weibo，JIA Shuyong，LI Hongyan
（1.School of Acu-moxibustion and Tuina，Beijing University of Chinese Medicine，Beijing 100029，China；2.Institute of Acu-moxibustion，China Academy of Chinese Medical Sciences，Beijing 100700，China）
Abstract: Objective: To establish a more stable pathological model of blocked channel by ligation in rats. Methods:48 male SD rats were randomly divided into 4 groups (n = 12). The Ren meridian (RN) low resistance line was measured first. Ligation group 1 ligated 1 point on the RN line; ligation group 2 ligated 2 point on the RN line; ligation group 3 ligated 3 point on the RN line; Ligation group 4 ligated 1 point out of the RN line, the ligation width was 1cm, and the ligation interval was 1-2mm. The interstitial fluid pressure wave propagation method was used to measure the amplitude of the interstitial fluid pressure wave propagation of the groups before and after the ligation. Results:The change rate of interstitial fluid pressure wave before and after ligation was 8.17%, -7.83%, -13.27% and -52.80% respectively in four groups.There was no significant difference between the ligation group 4, the ligation group 1 and the ligation group 2 after ligation, but the change rate of the three groups increased from positive to negative. The amplitude of the ligation group 3 was significantly reduced (P<0.01) after ligation compared to before ligation. Conclusion:The ligation method can establish a stable pathological model of channel blockage.
Key words: Meridian;Ligation; Channel Blockage; Rats; Interstitial Fluid Pressure Wave