Effect of triple energizer acupuncture method on the opening of mitochondrial membrane channel pore and apoptosis in dementia rats
FUYu1， WANG Liang2, XING Jing3， GUO Ruijing4， HAN Xiudi2
(1. The First Teaching Hospital of Tianjin University of TCM, Tianjin 300193,China 2. Tianjin University of TCM, Tianjin 300193,China；3.The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150009,China;4.Tianjin Huanhu Hospital, Tianjin 300193,China；)
Abstract: Objective: This eassy is about the effect of triple energizer acupuncture on the opening of mitochondrial membrane channel pore (mPTP) in dementia rats, and the effect of triple energizer acupuncture on neuronal apoptosis of hartz. Materials and Methods: Ⅱ The value of mouse cortex and cortex mitochondria and the effect of triple energizer acupuncture on mitochondrial membrane channel were investigated by measuring relative fluorescence value (RFU). the apoptotic IHS values of hippocampus and picengzu cells were detected by TUNEL method. Results: Ⅱ Compared with the SAMR1 group, the RUF values of SAMP8 control group and non acupoint group decreased significantly, and there was no significant difference between the acupuncture group and SAMR1 group. Ⅱ Hippocampus: there was no significant difference in IHS score between each group, p> 0.05; The expression of apoptotic IHS in model group was higher than that in SAMR1 group, p> 0.05; Compared with the model group, the acupuncture group had a decreasing tendency, p > 0.05; Cortex: the score of apoptosis was more than SAMR1 group, p < 0.05; Non acupoint group was greater than SAMR1 group, p < 0.05; The acupuncture group was less than non acupoint group, p < 0.05; Compared with the model group, the acupuncture group had a decreasing tendency, p > 0.05; There was no significant difference in the other groups, p> 0.05. Conclusion:The triple energizer acupuncture can inhibit the apoptosis of hippocampal mitochondrial membrane channel and neuron apoptosis in dementia rats.
Keywords: acupuncture, triple energizer acupuncture method, mPTP , Alzheimer's disease, neuronal apoptosis