Effects of Scalp Penetration Acupuncture on the Expression of TLR4 and MyD88 Protein in Ischemic Brain Tissue of MCAO/R Rats
WU Mingjuan, SUN Xiaowei, SUN Zhongren, CHEN Yinghua, JIN Hong, LIUTingting, LIU Tao, ZHANG Hongwei
(1 Heilongjiang Academy of Chinese Medicine, Harbin 150036, China; 2 The First Affiliated Hospital of
Heilongjiang University of Chinese Medicine, Harbin 150040, China; 3 Heilongjiang University of Chinese
Medicine, Harbin 150040, China)
Abstract: Objective: Cerebral ischemia-reperfusion injur(CIRI) is a more complex pathophysiological process, has not yet entirely clear. Recent studies have shown that inflammatory response is one of the important pathologies of CIRI.Which as the inflammatory response trader TLR4/MyD88/NF-κB cell signaling pathway all the key signal molecules can directly or indirectly affect the occurrence and the development of inflammation of the body,.In this study, we observed the effect of Scalp penetration acupuncture on the expression of TLR4 and MyD88 protein in the ischemic brain tissue of rats with cerebral ischemiareperfusion injury, and the neurological deficits, the infarct volume, the cortical neurons of cerebral ischemia- reperfusion model rats, To investigate the Neuroprotective effects of scalp penetration acupuncture on cerebral ischemia-reperfusion model rats and its possible mechanisms.Methods:99 Sprague-Dawley rats were randomly divided into sham operation group, model group and acupuncture group. According to the reperfusion time were divided into IR1d (n=13), IR3d (n=10) and IR7d (n=10) three subgroups. Using a modified Zea Longa suture method, preparation 2h right middle cerebral artery ischemia-reperfusion model (MCAO/R) rats;the sham operation group cerebral artery blood flow is not interrupted. Acupuncture group in the right side of the head Baihui to Qu Bin points line for Penetrating acupuncture treatment. The neurological score of Belayev's 12-point score was determined at the corresponding time points in each group; TTC staining was used to detect the cerebral infarct volume at 1 day after reperfusion; HE staining was used to observe the damage of cortical neurons and infiltration of inflammatory cells; Immunohistochemistry and Western blot were used to detect the expression of TLR4 and MyD88 protein in ischemic brain tissue of rats in each group.Results:1.The degree of neurological deficit was significantly lower in the acupuncture group than that in the model group at 3d and 7 days (P<0.05). 2.The volume of relative cerebral infarction in acupuncture group was significantly lower than that in model group, There are statistical differences.(P<0.05).3. reperfusion various time points, The degree of neuronal damage and inflammatory cell infiltration in the ischemic cortex of the acupuncture group were significantly lower than those in the model group. 4. acupuncture group ischemic brain tissue and the relative number of positive cells TLR4 protein content at various time points after reperfusion period compared with the model group significantly decreased (P<0.05), while the number of positive cells MyD88 and relative protein content in reperfusion 3d, 7d was significantly lower than the model group over the same period.(P<0.05). Conclusion: The treatment of Scalp penetration acupuncture can significantly improve the neurological deficit of MCAO/R rats, reduce the infarct volume, reduce the damage of cortical neurons, inflammatory cell infiltration, and have the protective effect of brain;The mechanism may be the Scalp penetration acupuncture can inhibit TLR4,MyD88 expression in the ischemic brain tissue, thus blocking the signal transduction of TLR4/MyD88/NF-κB cell signaling pathway, and then antagonizing the pathway triggered by the inflammatory cascade.
Key words: Acupuncture; cerebral ischemia-reperfusion injury; myeloid differentiation factor 88; Toll-like