WANG He, YANG Fangyuan, ZHAN He, GUO Qiulei, HONG Xinxun , CUI Zhiyuan, LIU Qingguo

( School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China )

Abstract：Objective: To explore the effect difference between twirling-rotating reinforcing and reducing technique of acupuncture on Cerebellum NE and 5-HT in Spontaneously Hypertensive rats (SHR) andits protectivemechanism.Methods:  Fifty male 9-week-old SHR rats were randomly  divided into 4 groups: normal  group,  model  group,  needle  retaining  group,  twirling-rotating  reinforcing  group,twirling-rotating reducing group, 10 per group.Acupuncture was not used in normal and control group,only with grasp,capture and binding  stimulation that  was  also adapt  in the  rest  groups.  Needle  retaining group was  treated  with acupuncture  at  “Taichong“  (LR3  )  without  any  technique  and  retaining  for  10  min  .Twirling-rotating reinforcing group and twirling-rotating reducing group were treated with Twirling-rotating reinforcing and reducing techniques  respectivelyat   (LR3 ) and retaining for 10 min. Before and after acupuncture, blood pressure  monitor  was  used  to  measure  the  blood  systolic  pressure.  28  days  after  treatment,  taking  the cerebellum,  Elisa was used to detect norepinephrine and serotonin in  the cerebellum. Results:  The systolic blood pressure of the model rats wassignificantly higher than that of the normal group  (P<0.05). Since 3 days  after  treatment,the  blood  pressure  in  needle  retaining  group,  twirling-rotating  reinforcing group,twirling-rotating  reducing  groupdecreased  than  that  of  model  group  (P  <0.05);Compared  with  the normal group, the content of NE and 5  -  HT in the cerebellum of model group, needle retaining group, twirling-rotating  reinforcing  group,twirling-rotating  reducing  groupwere  significantly  increased  (P <0.05);Compared  with  model  group,  the  contents  of  NE  and  5-HT  in  needle  retaining  group,  twirlingrotating  reinforcing  group,twirling-rotating  reducing  group  were  significantly  decreased  (P <0.05);Compared with needle and twirling-rotating reinforcing group,twirling-rotating reducing group NE decreased  significantly  (P<0.05). Conclusion:  Acupuncture  twirling-rotating  reinforcing  and  reducing technique can effectively reduce the systolic blood pressure of SHR rats. The antihype rtensive effect of acupuncture  and  diarrhea  was  related  to  the  contents  ofnorepinephrine(NE)  and  5-HT  (5-HT),  and  the regulation effect of twirling-rotating reinforcing and reducing technique were different.