Effects of Acupuncture and Moxibustion on Differentially Expressed Genes Numb1, Numb2 of Notch Signaling Pathway-related in Bone Marrow Hematopoietic Cells in Cyclophosphamide-induced Mice
LU Mei, DU Xue yuan, TENG Ying chun, LI Jian wei, ZHAO Xixin, CAO Da ming
(1Henan University of Chinese Medicine, No.156, Jinshui East RD, Zhengzhou 450046,China; 2The First Affiliated Hospital of Henan University of CM, Zhengzhou 450003,China;
3The Third Affiliated Hospital of Henan University of CM, Zhengzhou 450008,China)
Abstract: Objective: To explore the molecular biology mechanism underlying acupuncture and moxibustion in improving bone marrow suppression and periphery leucocyte by observation of effects of acupuncture and moxibustion on differentially expressed genes numb1, numb2 of Notch signaling pathway-related in bone marrow hematopoietic cells in cyclophosphamide-induced mice. Materials and Methods: 40 male Kunming mice(class SPF) were randomly divided into control group (group A), model group (group B), acupuncture group (group C) and moxibustion group (group D). The models of myelosuppression in group B, C and D were induced by cyclophosphamide, group A was given the same dose of saline. The protein and mRNA expression levels of differentially expressed genes numb1, numb2 of Notch signaling pathway-related were detected by immunohistochemical method, RT -PCR and Western-bolt Test after being treated by acupuncture and moxibustion. Results: Genes numb1 and numb2 which belong to the Notch signaling pathway-related genes in mice bone marrow hematopoietic cells have a significant difference. Acupuncture and moxibustion can increase numb1 and numb2 mRNA expression, thereby promoting numb1 and numb2 protein content increased. Conclusion: Acupuncture and moxibustion can reduce bone marrow suppression caused by chemotherapy of CTX, improve
hemopoietic function. One of its micromechanism could be: Acupuncture and moxibustion can increase the expression of Notch signaling pathway-related genes numb1, numb2, thus inhibiting the excessive activation of Notch signaling pathway. So that the bone marrow hematopoietic function was enhanced.
Key words: Acupuncture and moxibustion, Cyclophosphamide, Bone marrow suppression, Notch signaling pathway , Numb-Notch signaling pathway