Regulation of Acupuncture and Moxibustion on MDA and SOD in the Kidney of DDP-induced Mice
YU Dongdong, YAO Tiantian, LU Mei, YANG Jiaman
(1 Acupuncture and Moxibustion College, Henan University of Chinese Medicine, Zhengzhou, Henan, 450046)
Abstract: Objective: To investigate the effects of acupuncture and moxibustion on the expression of malondialdehyde (MDA) and superoxide dismutase (SOD) in kidney of mice after cisplatin chemotherapy (DDP). Methods: Forty clean male Kunming mice weighing 20 ± 2g were randomly divided into blank group, model group, acupuncture group and moxibustion group, with 10 rats in each group. Mice in model group, acupuncture group and moxibustion group were intraperitoneally injected with cisplatin 0.01ml/g (10mg/kg) one day before treatment according to body weight. Blank group were intraperitoneally injected with 0.9% Nacl solution in equal dosage. After successful modeling, acupuncture group and moxibustion group were given acupuncture, moxibustion treatment on "Dazhui", "Gan Yu", "Shenshu", "Zusanli" once a day. Model group and blank group were accompanied and fixed at the same time without any further treatment for 5 consecutive days. The contents of MDA and SOD in kidney were detected by ELISA. Results: Compared with the blank group, the content of MDA and SOD in the kidney of model group were significantly decreased (P <0.05) after 5 days of treatment. Compared with the model group, the content of MDA and the level of SOD in the liver of mice in acupuncture group and moxibustion group were significantly decreased (P <0.05) (P <0.05). Conclusion: Acupuncture and moxibustion can significantly inhibit the expression of MDA in the kidney of DDP model mice and promote the expression of SOD in the kidney tissue of DDP model mice, which has a protective effect on the kidney.
Key words: Acupuncture and Moxibustion, Cisplatin Chemotherapy , Kidney, Malondialdehyde, Superoxide Dismutase