Preliminary Study on Establishment of Pathological Channel Blockage Model in Rats by Ligation
HUANG Yan1，WANG Yanping1，ZHANG Weibo2△，JIA Shuyong2，LI Hongyan2
（1.School of Acu-moxibustion and Tuina，Beijing University of Chinese Medicine，Beijing 100029，China；2.Institute of Acu-moxibustion，China Academy of Chinese Medical Sciences，Beijing 100700，China）
Abstract: Objective: To establish a more stable pathological model of blocked channel by ligation in rats. Methods:48 male SD rats were randomly divided into 4 groups (n = 12). The Ren meridian (RN) low resistance line was measured first. Ligation group 1 ligated 1 point on the RN line; ligation group 2 ligated 2 point on the RN line; ligation group 3 ligated 3 point on the RN line; Ligation group 4 ligated 1 point out of the RN line, the ligation width was 1cm, and the ligation interval was 12mm. The interstitial fluid pressure wave propagation method was used to measure the amplitude of the interstitial fluid pressure wave propagation of the groups before and after the ligation. Results:The change rate of interstitial fluid pressure wave before and after ligation was 8.17%, -7.83%, -13.27% and -52.80% respectively in four groups.There was no significant difference between the ligation group 4, the ligation group 1 and the ligation group 2 after ligation, but the change rate of the three groups increased from positive to negative. The amplitude of the ligation group 3 was significantly reduced (P<0.01) after ligation compared to before ligation. Conclusion:The ligation method can establish a stable pathological model of channel blockage.
Key words: Meridian;Ligation; Channel Blockage; Rats; Interstitial Fluid Pressure Wave