LIU Xia, ZHONG Huan, LIU Qiong, LIU Tao, CAO Jianan, ZHANG Chi, CHANG Xiaorong

(1 College of Acu-moxibustion and Massage, Hunan University of Chinese Medicine, Changsha 410007, China) 

Abstract:Objective: 1. T o  observe  the effects of moxibustion and acupuncture on local point area organization immune cells, immune factors and metabolism in physiology and CAG pathological state;  2. T o reveal the regulation mechanism differences between Moxibustion and Acupuncture on local points area on physiology and CAG pathological.  Methods:  1. MNNG ally  multi-factor method replicate  CAG model rats, with acupuncture and moxibustion as intervention, set up the normal  group,  normal+moxibustion  group  and  normal+acupuncture  group,  model  group, model+moxibustion  group,  model+acupuncture  group,  according  to  the  subjects  factorsintervention groups, HE staining to observe each group rats gastric histopathological features;   2. The immunohistochemical method to detect the local  points  area tissue neuropeptide (SP, NPY, CGRP, slow excitation peptide), histamine receptor, inflammatory factor (IL-1 beta, IL -  6, IL -  10, IL  -  18, TNF alpha) content;3. By using 1  H  NMR detection of metabolites in local  points  area organization,  through  the  PCA,  PLS  -  DA,  OPLS  -  DA  analysis  methods  to  explore  the commonness  and  differences  between  Moxibustion  and Acupuncture.Results:  1.  "ST  36"  area number of mast cells in Model + moxibustion group and model+acupuncture group are more than 

model group, model plus moxibustion group was significantly higher than that of model group, the mean comparison (P<0.05,  the difference is statistically significant.  Acceptance of moxibustion or  normal  rats  foot  after  the  "ST  36"  acupuncture  intervention  zone  tissue  mast  cells  also  has increasing trend, moxibustion also has significant difference, P<0.05).2. The way of  moxibustion and  acupuncture  stimulation  of  local  "  ST  36"  hole  area  tissue  neuropeptide  expressions  have different influence, moxibustion on local "ST  36"  point  area organization NPY, slow excitation peptide  has  significant  effect  (P<0.05;  P<0.01),  and  the  change  in  the  CAG  pathological  and physiological state. "ST 36" acupuncture on local area organization CGRP and SP has significant effect (P<0.05; P<0.01), and the change in the CAG pathological and physiological state.  In the CAG  pathological  state  of  "ST  36"  local  hole  area  organization  is  in  a  state  of  inflammation, moxibustion can enhance the degree of inflammation, acupuncture can reduce the inflammatory state.3. The way of moxibustion and acupuncture stimulation of local "ST  36"  point  area tissue metabolism have different effects, moxibustion has affected the local tissue of energy metabolism, glycolysis, nucleic acid metabolism, taurine, choline phosphate metabolism.  Acupuncture mainly affects the local tissue of energy metabolism, amino acid metabolism, nucleic acid metabolism and glycolysis.  In the CAG pathological state local "ST  36"  point  area tissue metabolism disorders, mainly  displays  in  the  neurotransmitter  metabolic  disorders  and  energy  metabolism  disorders.After  moxibustion  intervention  mainly  adjust  the  local  hole  area  organization  disorders  of neurotransmitter, on energy metabolism also play a regulatory role;  After acupuncture intervention mainly  adjust  the  local  hole  area  organization  the  neurotransmitter  metabolic  disorder  state. Conclusion: 1. Moxibustion and acupuncture have different regulation effects on local points area organization   of immune cells, the commonness of moxibustion and acupuncture regulation  is promote  the  local  point  district  organization  the  accumulation  of  the  immune  cells,  and moxibustion effect is better than acupuncture, the difference has nothing to do with the body's disease states. 2. The generality of the physiological state of moxibustion and acupuncture in order to  promote  the  local  hole  area  of  tissue  inflammation  state,  members  of  the  opposite  sex  as proinflammatory role way different.  The pathological state of moxibustion and acupuncture for adjusting  the  local  points  area  organization  of  the  generality  of  the  inflammatory  state, heterosexual as the moxibustion as proinflammatory response, acupuncture tend to inhibition of inflammation.3.  Physiological  state  of  moxibustion  and  acupuncture  for  the  generality  of  the metabolic state of active, the opposite sex for the biological effect difference, moxibustion tend to promote the local tissue cells apoptosis, acupuncture tend to the neurotransmitter regulation. The pathological  state  of  moxibustion  and  acupuncture  for  the  generality  of  the  metabolic  state regulation of the opposite sex for the biological effect difference, moxibustion on local points area organizations disorder in the neurotransmitter and energy metabolism has a regulatory role, and acupuncture mainly control the local points  area disorders of neurotransmitter in the organizations.

Key Words:  Moxibustion; Acupuncture; Metabonomics; local point organization; Comparative study