HUANG Yan, WANG Yanping, JIA Shuyong, LI Hongyan, WANG Ze, YE Fengyao, WANG Guangjun, TIAN Yuying, ZHANG Weibo

（1 School of Acu-moxibustion and Tuina，Beijing University of Chinese Medicine，Beijing 100029；2 Institute of Acu-moxibustion，China Academy of Chinese Medical Sciences，Beijing 100700）

Abstract: Objective:  To establish a more stable pathological model of blocked channel by ligation in rats. Methods:  48  male  SD  rats  were  randomly  divided  into  4  groups  (n  =  12).  The  Ren  meridian  (RN)  low resistance line  was measured first.Ligation group 1ligated  1 point on the RN  line;ligation group 2ligated  2 point on the RN  line; ligation group3 ligated  3 point on  the RN  line;Ligation group 4ligated  1 point out of the RN line, the ligation width was 1cm, and the ligation interval was 1-2mm. The interstitial fluid pressure wave  propagation  method  was  used  to  measure  the  amplitude  of  the  interstitial  fluid  pressure  wave propagation of the groups before and after the ligation.  Results: The change rate of interstitial fluid pressure wave before and after ligation was 8.17%, -7.83%, -13.27% and -52.80% respectively  in four groups.There was no significant difference between the ligation group4, the ligation group 1and the ligation group 2after ligation, but the change rate of the three groups increased from positive to negative.The amplitude of the ligation group 3was significantly reduced (P<0.01) after ligation compared to before ligation.  Conclusion:The ligation method can establish a stable pathological model of channel blockage.

Key words: Acupunctureand moxibustion, Meridian, Ligated, Tissue fluid