ZHU Yi, LI Yi，ZHANG Nan, DONG Guirong

(Department of Acupuncture, Yueyang Hospital of Integrated Traditional Chinese Western Medicine, ShanghaiUniversity of Traditional Chinese Medicine，Shanghai, 200437，China)

Abstract： Objective：To observe the effect of electroacupuncture(EA) pretreatment on mRNA expression of TLR4、MyD88 and NF-кB of “Neiguan” Point（PC 6）in the PC 6 area of rats with myocardial  ischemia-reperfusion  injury.  Methods ：   The  healthy  wistar  rats  were  randomly divided into 6 groups, i.e., the control group, the normal EA group, the sham operation group ,the model group, the EA group, the sham EA group, 8 in each group. The rats in the EA group, the normal EA group and the sham EA group were treated with  EA (2~20 Hz in frequency, tolerated intensity of rats) at “Neiguan” (PC 6), 30 min per treatment, once a day for consecutive 5 days. The rats in the sham operation group and model group were treated with immobilization for the same  time,  and  no  EA  was  given. Animals  were  subjected  to  30  min  of  ischemia  induced  by occluding the left anterior descending artery followed by 120 min of  reperfusion after the end of EA. The mRNA expression of TLR4, MyD88 and NF-κB in the  PC6 area were determined with Real-time  PCR.  Results ：  Compared  with  the  normal  group,  the  ECG  J-point  height  were significantly increased in the model group (P<0.01)， compared with the model group and  the sham EA group ,EA preconditioning can  significantly  reduced ECG  -  J point height(P<0.01); The  mRNA expression of TLR4, MyD88 and NF-κB were significantly increased in the model group and the sham  EA  group  compared  with  the  control  group  (P<0.01). EA  preconditioning  significantly reduced the the mRNA expression of TLR4, MyD88 and NF-κB compared with the model group and the sham EA group (P<0.05).  Conclusion：  EA preconditioning significantly reduced the the mRNA expression of TLR4, MyD88 and NF-κB in the PC6 area of rats with myocardial ischemiareperfusion injury.

Key words: Acupuncture, The PC6 area, Ischemia-reperfusion injury, TLR4、MyD88、NF-кB