LU Yan, Shen Qing-si, Tian Meng,Zhang Jing, Zhang Yong-chen, Jia Hong-ling

(1.College of Acumox and Tuina, Shandong University of TCM, Jinan 250355, China；2. Master of Shandong University of TCM, Jinan 250355, China；4. The Second Affiliated Hospital of Shandong University of TCM, Jinan, 250014, China,)

Abstract: Objective: To investigate the mechanism of drug-separated moxibustion at the navel on vascular endothelial  growth  factor(VEGF)and  anti-Mullerian  hormone(AMH)  in  rats  with  premature  ovarian failure(POF) . Methods：50 female Wistar rats were randomly divided into normal group, model group,the group of herb-partion moxibustion at navel and Western medicine group. The rat model of premature ovarian failure were used by intraperitoneal injection of cyclophosphamide. The rats of herb-partion moxibustion at navel group were treated with self-made herb ointment. The rats in the western medicine group were givenintragastric dose of estradiol valerate. After 2 weeks of treatment, the levels of E2, LH and FSH were detected by  using  the  radioimmunoassay  method.  ELISA  was  used  to  detect  the  level  of  VEGF  and  AMH. Results:Compared with the normal group, the level of  E2 decreased in model group (P<0.05). The level of E2 in the group of herb-partion moxibustion at navel and Western medicine group were higher than that in the model group (P<0.05). Compared with the normal group, the level of VEGF and AMH significantly decreased  in  the  model  group  (P<0.05).  The  level  of  VEGF  and  AMH  in  the  group  of  herb-partion moxibustion at navel and Western medicine group were increased than those in the model group (P<0.05). Conclusion: The   herb-partion  moxibustion  at  navel  can  significantly  promote  the  follicle  number  and 

ovarian reserve of POF rats may be related to the regulation of E2, VEGF and AMH.

Key  words:  Herb-partition  moxibustion  at  navel;  Premature  ovarian  failure;  Sex  hormones;  Vascular endothelial growth factor; Anti Mullerian hormone