FUYu¹， WANG Liang², XING Jing³， GUO Ruijing⁴， HAN Xiudi² 

(1. The First Teaching Hospital of Tianjin University of TCM, Tianjin 300193,China 2. Tianjin University of TCM, Tianjin 300193,China；3.The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150009,China;4.Tianjin Huanhu Hospital, Tianjin 300193,China；)

Abstract: Objective:  This eassy is about the effect of triple energizer acupuncture on the opening of  mitochondrial  membrane  channel  pore  (mPTP)  in  dementia  rats,  and  the  effect  of  triple energizer acupuncture on neuronal apoptosis of hartz.  Materials and Methods: Ⅱ The value of mouse  cortex  and  cortex  mitochondria  and  the  effect  of  triple  energizer  acupuncture  on mitochondrial  membrane  channel  were  investigated  by  measuring  relative  fluorescence  value (RFU). the apoptotic IHS values of hippocampus and picengzu cells were detected by TUNEL method. Results: Ⅱ Compared with the SAMR1 group, the RUF values of SAMP8 control group and non acupoint group decreased  significantly, and there was no significant difference between the acupuncture group and SAMR1 group. Ⅱ Hippocampus: there was no significant difference in IHS score between each group,  p> 0.05; The expression of apoptotic IHS in model group was higher than that in SAMR1 group,  p> 0.05; Compared with the model group, the acupuncture group had a decreasing tendency, p > 0.05; Cortex: the score of apoptosis was more than SAMR1 group, p < 0.05; Non acupoint group was greater than SAMR1 group, p < 0.05; The acupuncture group  was  less  than  non  acupoint  group,  p  <  0.05;  Compared  with  the  model  group,  the acupuncture group had a decreasing tendency, p > 0.05; There was no significant difference in the other groups, p> 0.05. Conclusion:The triple energizer acupuncture can inhibit the apoptosis of hippocampal mitochondrial membrane channel and neuron apoptosis in dementia rats.

Keywords:  acupuncture,  triple  energizer  acupuncture  method,  mPTP ,  Alzheimer's  disease, neuronal apoptosis