CHEN Mei-lin, WANG Chao, TAN Cheng-fu, LIU Wei-wei, GUO Lina, DU 

Lin, TANG Ya-ni, LI Jiaolan, ZHU Dingming

(College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China) 

Abstract: Objective：To  observe  the  effect  of  electroacupuncture  (EA)  preconditionning  of  bilateral “Neiguan ”（PC6）on serum metabolism in myocardial ischemia reperfusion injury (MIRI) rats by nuclear magnetic  resonance  spectroscopy  (1H  NMR)  technology  .  Methods：48  male  SD  rats  were  randomly divided into blank control, model, EA PC6 and EA “Hegu”(LI4) groups (n=12/ group).The Control group was trussed up for 30 min,once daily foy 7 days . The model group was trussed up as the control group and ligated the left anterior descending coronary artery for 40 min, restored the blood flow for 1 h to replicate the MIRI model.EA preconditionning was respectively applied to bilateral PC6 and (LI4) for 30 min,once daily foy 7 days,to 7th day replicate the MIRI model. Collecting the serum of rats in each group, checking the  metabolin  in  serum  by  (1H  NMR)  technology  and  discriminating  the  metabolic  model.  Results：Compared with the control group, the model of serum metabolism in model group was changed obviously, compared with the model group, the model of serum metabolism in EA PC6 group was changed obviously and  Glucose increased， Leucine， Isoleucine， Valine， 3-HBA， Lactate， Acetate， Acetone， Acetoacetate，Pyruvate，Glutamine，Creatine，Glycerol decreased. Conclusion：EA preconditionning PC6 can play a 

certain pre protection role to MIRI rats by regulating the gluconeogenesis, pyruvate metabolism,amino acid metabolism, ketone body metabolism and energy metabolism.

Key words: Electroacupuncture preconditionning; Myocardial ischemia-reperfusion; Metabonomics